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KMID : 0545120010110020302
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 2 p.302 ~ p.309
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Enumeration of Vibrio vulnificus in Natural Samples by Colony Blot Hybridization
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Lee, Jeong Hyun
Lee, Kyu Ho/Choi, Sang Ho
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Abstract
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Colony blot hybridization using a VVHP DNA probe derived from the sequence of the hemolysin gene, vvhA, was specific in identifying all V vulnificus strains, thereby, eliminating the need for any additional phenotypic identification. The colony blot hybridization procedure revealed a sensitivity and broad applicability sufficient for the direct enumeration of V vulnificus in various natural samples, without the use of enrichment or culturing on selective media. V vulnificus was detected in all natural samples collected during August and May at concentrations ranging from 2.1×10 exp(1) to 4.O×10 exp(3) organisms per ml. However, during November and February, when the mean temperatures of the seawater were 12℃ and 5℃, respectively, V vulnificus was not detected in any natural samples.
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